Virtual Reality / Plant Science & Biotechnology
Extraction of Dna From Plant Material VR Simulation Training
Overview
Extraction of Dna From Plant Material VR Simulation Training by ASFAN gives learners focused, repeatable practice through immersive scenarios that support skill development, assessment, and safer preparation.
Materials Provided in VR
- Fresh plant tissue (~1 g).
- Mortar and pestle; optional liquid nitrogen for hard tissues.
- CTAB-based extraction buffer (2% CTAB, Tris-HCl, EDTA, NaCl, optional -mercaptoethanol).
- Chloroform:isoamyl alcohol (24:1).
- Cold isopropanol and 70% ethanol.
- TE buffer.
- Microcentrifuge tubes and centrifuge.
- Heat block or water bath (65C).
Learning Outcomes
- Observe DNA extraction steps in real time, from tissue lysis to DNA precipitation.
- Understand the role of buffers, alcohols, and centrifugation in isolating DNA.
- Practice precise lab techniques and aseptic handling virtually.
- Recognize challenges with plant polysaccharides and learn strategies to overcome them.
Step-by-Step VR Procedure
1
Grind Plant Tissue
Freeze (optional) and grind tissue to a fine powder.
2
Lyse Cells
Mix powder with pre-warmed CTAB buffer and incubate at 65C.
3
Remove Proteins & Debris
Add chloroform:isoamyl alcohol, mix gently, and centrifuge.
4
Isolate DNA
Transfer the aqueous layer, add cold isopropanol, and incubate to precipitate DNA.
5
Pellet DNA
Centrifuge to collect DNA, discard supernatant.
6
Wash DNA
Rinse pellet with 70% ethanol, centrifuge, and air-dry.
7
Resuspend DNA
Dissolve in TE buffer or nuclease-free water; store at -20C.
Key Advantages of VR
- Safe and repeatable training for molecular biology Simulation Training Modules.
- Visualization of otherwise invisible processes like DNA precipitation.
- Encourages hands-on learning and scientific accuracy without chemical hazards.
- Suitable for school labs, STEM programs, and remote learning environments.
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- Immersive, interactive VR labs that boost learner engagement.
- Safe, repeatable simulations without fragile equipment.
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