Virtual Reality / Plant Science & Biotechnology

Extraction of Dna From Plant Material VR Simulation Training

Overview

Extraction of Dna From Plant Material VR Simulation Training by ASFAN gives learners focused, repeatable practice through immersive scenarios that support skill development, assessment, and safer preparation.

Extraction of Dna From Plant Material VR Simulation Training

Materials Provided in VR

  • Fresh plant tissue (~1 g).
  • Mortar and pestle; optional liquid nitrogen for hard tissues.
  • CTAB-based extraction buffer (2% CTAB, Tris-HCl, EDTA, NaCl, optional -mercaptoethanol).
  • Chloroform:isoamyl alcohol (24:1).
  • Cold isopropanol and 70% ethanol.
  • TE buffer.
  • Microcentrifuge tubes and centrifuge.
  • Heat block or water bath (65C).

Learning Outcomes

  • Observe DNA extraction steps in real time, from tissue lysis to DNA precipitation.
  • Understand the role of buffers, alcohols, and centrifugation in isolating DNA.
  • Practice precise lab techniques and aseptic handling virtually.
  • Recognize challenges with plant polysaccharides and learn strategies to overcome them.
Step-by-Step VR Procedure
1

Grind Plant Tissue

Freeze (optional) and grind tissue to a fine powder.

2

Lyse Cells

Mix powder with pre-warmed CTAB buffer and incubate at 65C.

3

Remove Proteins & Debris

Add chloroform:isoamyl alcohol, mix gently, and centrifuge.

4

Isolate DNA

Transfer the aqueous layer, add cold isopropanol, and incubate to precipitate DNA.

5

Pellet DNA

Centrifuge to collect DNA, discard supernatant.

6

Wash DNA

Rinse pellet with 70% ethanol, centrifuge, and air-dry.

7

Resuspend DNA

Dissolve in TE buffer or nuclease-free water; store at -20C.

Key Advantages of VR

  • Safe and repeatable training for molecular biology Simulation Training Modules.
  • Visualization of otherwise invisible processes like DNA precipitation.
  • Encourages hands-on learning and scientific accuracy without chemical hazards.
  • Suitable for school labs, STEM programs, and remote learning environments.
Extraction of Dna From Plant Material VR Simulation Training

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