VR Micropropagation: Shoot Tip Culture

Overview

This VR module guides students through shoot tip culture, a micropropagation technique used to produce virus-free plants and rapidly multiply desirable genotypes. Learners practice excising shoot tips, aseptic inoculation, and plantlet development in a fully virtual lab.

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Learning Outcomes

Understand micropropagation principles and the use of shoot tips for plant multiplication.
Practice aseptic techniques and precise handling in VR.
Observe the effect of growth regulators on shoot induction and proliferation.
Learn how virus-free plants are produced and multiplied efficiently.

Materials Provided in VR

Shoot tip explants (apical meristems).
Sterile scalpels and forceps.
Murashige & Skoog (MS) medium with cytokinins and auxins.
Culture tubes or Petri dishes.
Laminar flow hood (virtual) for aseptic manipulation.
Growth chamber with controlled light, temperature, and humidity.

Step-by-Step VR Procedure

Explant Selection

Choose healthy apical shoot tips from donor plants.

Surface Sterilization

Disinfect shoot tips to remove microbial contaminants.

Inoculation

Place sterilized shoot tips onto prepared MS medium under sterile conditions.

Incubation & Growth

Maintain cultures in controlled conditions to promote shoot multiplication.

Observation & Subculture

Monitor shoot growth and transfer to fresh medium for further multiplication.

Key Benefits of VR

Safe, repeatable practice without contamination risks.
Real-time visualization of shoot tip growth and plantlet development.
Reinforces scientific protocols and sterile handling.
Suitable for schools, universities, and plant biotechnology training.

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