Virtual Reality / Plant Science & Biotechnology
Micropropagation Axillary Bud Culture VR Simulation Training
Overview
Micropropagation Axillary Bud Culture VR Simulation Training by ASFAN gives learners focused, repeatable practice through immersive scenarios that support skill development, assessment, and safer preparation.
Learning Objectives
- Understand axillary bud culture as a clonal propagation technique.
- Practice aseptic dissection of nodal segments and bud isolation.
- Prepare and use culture media for bud initiation and shoot elongation.
- Manage contamination risks and maintain sterile workflow.
- Track shoot development and prepare plantlets for acclimatization.
Materials Provided in VR
- Nodal segments with axillary buds.
- Sterile scalpel, forceps, and culture vessels.
- Murashige & Skoog (MS) media with hormones for shoot induction.
- Laminar flow hood (virtual) for aseptic transfers.
- Growth chamber with controlled light and temperature.
Step-by-Step VR Procedure
1
Select & Prepare Explant
Choose healthy nodal segments containing axillary buds; trim to size.
2
Surface Sterilization
Sterilize explants to remove microbes without damaging tissues.
3
Inoculation on Medium
Place sterilized nodes on hormone-supplemented MS medium.
4
Shoot Induction
Maintain cultures to promote bud break and shoot elongation.
5
Rooting & Acclimatization
Transfer shoots to rooting medium, then prepare plantlets for hardening.
Key Benefits of VR
- Safe, repeatable micropropagation training without live contamination risks.
- Hands-on practice with sterilization, inoculation, and shoot induction workflows.
- Clear visualization of bud break, shoot elongation, and rooting stages.
- Ideal for teaching clonal propagation in plant biotechnology curricula.
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- Immersive, interactive VR labs that boost learner engagement.
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