VR Micropropagation: Axillary Bud Culture

Overview

This VR module guides learners through axillary bud culture, a reliable micropropagation method that multiplies plants from existing shoot meristems. Students practice sterile dissection, media preparation, and bud induction in a fully interactive virtual lab.

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VR micropropagation axillary bud culture

Learning Objectives

Understand axillary bud culture as a clonal propagation technique.
Practice aseptic dissection of nodal segments and bud isolation.
Prepare and use culture media for bud initiation and shoot elongation.
Manage contamination risks and maintain sterile workflow.
Track shoot development and prepare plantlets for acclimatization.

Materials Provided in VR

Nodal segments with axillary buds.
Sterile scalpel, forceps, and culture vessels.
Murashige & Skoog (MS) media with hormones for shoot induction.
Laminar flow hood (virtual) for aseptic transfers.
Growth chamber with controlled light and temperature.

Step-by-Step VR Procedure

Select & Prepare Explant

Choose healthy nodal segments containing axillary buds; trim to size.

Surface Sterilization

Sterilize explants to remove microbes without damaging tissues.

Inoculation on Medium

Place sterilized nodes on hormone-supplemented MS medium.

Shoot Induction

Maintain cultures to promote bud break and shoot elongation.

Rooting & Acclimatization

Transfer shoots to rooting medium, then prepare plantlets for hardening.

Key Benefits of VR

Safe, repeatable micropropagation training without live contamination risks.
Hands-on practice with sterilization, inoculation, and shoot induction workflows.
Clear visualization of bud break, shoot elongation, and rooting stages.
Ideal for teaching clonal propagation in plant biotechnology curricula.

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