Virtual Reality / Plant Science & Biotechnology
VR Micropropagation: Anther Culture
Overview
This VR module introduces students to anther culture, a micropropagation technique used to produce haploid or doubled-haploid plants. Learners practice excising anthers, aseptic inoculation, and callus or embryo induction in a fully virtual lab environment.
Materials Provided in VR
- Anther explants from donor plants.
- Sterile scalpels, forceps, and Petri dishes.
- Murashige & Skoog (MS) medium supplemented with appropriate hormones.
- Laminar flow hood (virtual) for sterile manipulation.
- Growth chamber with controlled light, temperature, and humidity.
Learning Outcomes
- Understand principles of anther culture and haploid/doubled-haploid plant production.
- Practice aseptic techniques and precise tissue handling in VR.
- Observe callus/embryo formation and learn how plantlets regenerate.
- Gain insight into plant breeding and genetic improvement techniques.
Step-by-Step VR Procedure
1
Explant Selection
Select healthy anthers at the correct developmental stage.
2
Surface Sterilization
Disinfect anthers to eliminate microbial contamination.
3
Inoculation
Place sterilized anthers onto prepared MS medium under aseptic conditions.
4
Incubation & Induction
Maintain cultures to induce callus or embryoid development.
5
Observation & Regeneration
Monitor development and transfer tissues to fresh medium for shoot and root formation.
Key Benefits of VR
- Safe, repeatable micropropagation practice.
- Real-time visualization of anther growth, callus, and plantlet regeneration.
- Reinforces laboratory protocols, sterile handling, and plant biotechnology concepts.
- Ideal for schools, universities, and advanced plant science training.
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